Specifically, U87MG cells have a mean doubling time of 30.8 ± 2.5 h, which is the slowest division between the cell types we use. Among the primary cell lines, GBP03 cells divide approximately every 25.4 ± 0.5 h, while GBP06 and GBP08 have similar doubling times estimated at 23.5 ± 0.7 h and 23.0 ± 1.5 h, respectively. 3.2.
12/18/2017 · Allen M, et al. Origin of the U87MG glioma cell line: Good news and bad news. Sci. Trans. Med. 8(354): 1-4, 2016. Clark MJ, et al. U87MG decoded: The genomic sequence of a cytogenetically aberrant human cancer cell line. PLoS Genetics 6 (1) : e1000832, 2010.
Population Doubling Time (PDT) is approximately 34 h (Appendix 2). Page . 3 . of . 24, 4/22/2002 · Four weeks after the initiation of the treatment, the tumour doubling time in animals treated with AN-215 was significantly prolonged from 4.5±0.2 to 8.2±1.8 days (P doubling time in mice given an equimolar dose of AN-201 was 5.9±1.4 days which did not differ significantly from the controls.
I’ve also tried longer trypin treatment time (20min at 37C), which seemed to help the cells to spread out nicely on the 2nd day, however, the problem came back on the 4th day. 7.
1/29/2010 · Author Summary Glioblastoma has a particularly dismal prognosis with median survival time of less than fifteen months. Here, we describe the broad genome sequencing of U87MG , a commonly used and thus well-studied glioblastoma cell line. One of the major features of the U87MG genome is the large number of chromosomal abnormalities, which can be typical of cancer cell lines and primary.
History and Characteristics. Formally known as U-87 MG (abbreviation for Uppsala 87 Malignant Glioma), the U87 cell line has an epithelial morphology and was obtained from a 44-year-old female patient in 1966 at Uppsala University.The cell line was thought to be deposited at the Memorial Sloan Kettering Cancer Center in 1973, after which the ATCC obtained it in 1982.
However, determination of doubling time between the two cell lines showed very similar results, i.e.
29.2 h for U87MG and 29.9 h for T98G. Figure 2 Functional analysis of.
For U87MG cells I have had great luck culturing them in MEM-alpha with 10% FBS and antibiotics. I have yet to notice a change in their growth rate, even after 20-30 passages or when I create …
B) Doubling time of T98G, U373MG and U87MG calculated as the average of doubling time during days of colture. C) Facs analysis of T98G, U373MG and U87MG gl ioblastoma cell cycle.
